Anachronisms
During the opening musical number, Big Daddy holds up a newspaper while in the car revealing a number of headlines including "Ford Corporation Introduces Trimotor" and "Gertrude Ederle Readies to Swim the English Channel." Neither of these events, nor Mardi Gras for that matter, took place on the newspaper's supposed date of April 25th 1926. The Trimotor first flew in June 1926, Ederle wouldn't swim the English Channel until August 6th, and Mardi Gras in 1926 fell on February 16th.
There are at least two references to "Bananas Foster," however this dish wasn't introduced until 1951.
Audio/visual unsynchronised
When Lou tells Naveen "No." after listing the many threats of the bayou, it does not match up with his lips moving on the screen. The sound is just a little bit late.
When Dr. Facilier steps on Ray, the sound indicates that the bug is crushed. A little later, Ray is shown still alive (though just barely) and in one piece.
Continuity
When Prince Naveen prepares the romantic dinner, he leans over the improvised table, and falls. However, only seconds later he gets up and sits on the edge of the table, which this time supports his full weight.
After Dr. Facilier retrieves the talisman from Tiana he turns her back into a frog and pins her down with his cane. The cane is there but after she grabs the talisman with her tongue the cane disappears and is nowhere to be found.
During the song "Friends on the other side" Dr Facilier spreads the cards from right to left. After a cut to Prince Naveen's face the cards have switched directions as if spread from left to right allowing him to flip them over the way he did.
The first time you see Tiana swaying with the music Charlotte and Naveen are dancing to, she isn't wearing any earrings. The second time you see her swaying, however, she has a pair on.
The first time the brunette and the blonde are seen clapping along with Naveen's performance, the brunette is wearing a lot of jewelry. However, the second time they are shown, some of the jewelry is gone.
In the scene where Tiana is debating whether or not to kiss the frog there is a portrait on the back wall the keeps on disappearing and reappearing.
When Charlotte kisses Prince Naveen as a frog, toward the end of movie, he is covered with lipstick kisses, but in the next shot the lipstick is gone.
Towards the end of the movie when Tiana is building her restaurant, the building on the left slides over to the left in the last flash scene.
The newspaper at the beginning of the movie talks about Naveen's trip to the states and reports that the small obscure country sent their only son. At the end of the movie in Tiana's Palace Charlotte is dancing with Naveen's six and half year old brother.
Errors in geography
In the final scene you see the Mississippi River behind St. Louis Cathedral. In actuality the Mississippi River is in front of the Cathedral.
Factual errors
Louis is established to be an alligator in the film. But when an alligator's mouth is closed, its teeth do not show out like a crocodile's. In the shots where Louis's mouth is closed, his teeth are showing.
Incorrectly regarded as goofs
Tiana is left-handed, but sometimes uses her right hand for writing and kitchen work. For much of European and American history, society considered using the left hand to be sinful or unnatural, so "lefties" often felt compelled to use the right hand in polite company.
The fireflies consider themselves Cajun, but play Creole music. Human definitions and divisions are not required to apply to insects.
On several occasions (particularly in "Almost There" and at the ball), there are numerous people either drinking or holding glasses of wine or champagne. This movie is set primarily in the 1920s. The possession and sale of alcohol in the United States was illegal between 1919 and 1933, because of the 18th Amendment (Prohibition), though Prohibition was widely disregarded, especially in New Orleans.
In the beginning of the movie, Tiana's dad writes "Tiana's PLACE" on the picture. At the end, the menu reads "Tiana's PLACE", but the restaurant is named "Tiana's PALACE". This deliberate name change is to emphasize the fact that Tiana is now a princess.
Revealing mistakes
When Naveen is mincing a mushroom for Tiana, you can see that his fingers are clearly in the way of the knife, yet he does not cut himself when he slices through the mushroom.